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Time-of -addition experiments in Huh7 cells exposed that tomatidine acts at a publish-entry stage of the virus replication cycle. Additionally, a marked lessen in the number of CHIKV-contaminated cells was viewed, suggesting that tomatidine predominantly functions early in an infection still just after virus attachment and cell entry. Antiviral exe
Eventually, 1 μCi of 86Rb was included to each lifestyle and incubation was continued at 37 °C for an additional 10 min. Reactions were terminated by washing the cells with ice-cold one hundred mM MgCl2 and cell lysates were prepared for liquid scintillation counting. The 86Rb counts in parallel cultures with ouabain had been subtracted f